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Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay

Identifieur interne : 001433 ( Main/Exploration ); précédent : 001432; suivant : 001434

Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay

Auteurs : Jin Yang [République populaire de Chine] ; Mei-Xin Fang [République populaire de Chine] ; Jie Li [République populaire de Chine] ; Guo-Qiang Lou [République populaire de Chine] ; Hang-Jun Lu [République populaire de Chine] ; Nan-Ping Wu [République populaire de Chine]

Source :

Archives of Virology [ 0304-8608 ] ; 2011-08-01.

RBID : ISTEX:21D8CBE0F8B3F3E1EFAD588B4E37655B3D6BE42C

Abstract

Abstract: An improved, sensitive, specific, and rapid one-step reverse transcription loop-mediated isothermal amplification (LAMP) assay targeting the 5′ untranslated region (UTR) was developed to detect hepatitis C virus (HCV) infection. Based on an accelerating primer (AP), the present assay, named AP-LAMP, has the advantages of rapidity and sensitivity over the routine LAMP method. The possible AP-based amplification pathway during the reaction was revealed by restriction enzyme digestion and eletrophoresis. The detection limit of the AP-LAMP assay was approximately 84 IU/ml, and no cross-detection was observed. The assay was evaluated further with 126 clinical specimens, and the results indicated the suitability and simplicity of the test as a rapid diagnostic tool for detection of HCV RNA.

Url:

DOI: 10.1007/s00705-011-1001-4

Affiliations:

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<front><div type="abstract" xml:lang="en">Abstract: An improved, sensitive, specific, and rapid one-step reverse transcription loop-mediated isothermal amplification (LAMP) assay targeting the 5′ untranslated region (UTR) was developed to detect hepatitis C virus (HCV) infection. Based on an accelerating primer (AP), the present assay, named AP-LAMP, has the advantages of rapidity and sensitivity over the routine LAMP method. The possible AP-based amplification pathway during the reaction was revealed by restriction enzyme digestion and eletrophoresis. The detection limit of the AP-LAMP assay was approximately 84 IU/ml, and no cross-detection was observed. The assay was evaluated further with 126 clinical specimens, and the results indicated the suitability and simplicity of the test as a rapid diagnostic tool for detection of HCV RNA.</div>
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Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay

Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay

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Abstract

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